I can't tell you how many women come into my office fretting about the fact that they've over-plucked. While this is certainly a possibility (plucking the eyebrows damages the hair follicle, and with repeated plucking, the follicle can get damaged to the point that it can no longer produce a hair), there are actually a number of both external and internal causes and conditions that can lead to temporary or permanent loss of eyebrows.
Reduce stress. When you are stressed, your body starts shutting down processes that aren’t necessary for survival. This shutdown can occur with physical stressors, like surgery or an illness, and emotional stress, which often manifests as physical symptoms. Loss may occur up to three months after the stressful event and may take another three to grow again.[24]
Androgenetic alopecia, a type of hair loss commonly called male or female pattern baldness, was only partially understood until the last few decades. For many years, scientists thought that androgenetic alopecia was caused by the predominance of the male sex hormone, testosterone, which women also have in trace amounts under normal conditions. But while testosterone is at the core of the balding process, dihydrotestosterone (DHT) is now thought to be the main culprit.

DLE is an autoimmune condition and is the most common form of chronic cutaneous lupus erythematosus.[35] Clinically, the lesions start as discoid erythematous patches which then develop into plaques with follicular plugging and scaling. Eyelid findings include blepharitis, lid scarring, entropion, and ectropion. Scaly plaques on the eyelids with loss of hair follicles results in madarosis[60] [Figure 3]. Numerous studies have reported the mimicking of a chronic blepharitis by DLE.[35,61–63] A high index of suspicion is necessary in such cases, where the diagnosis is very often delayed by months to years.[35] Biopsy with histopathological examination should be done to confirm the diagnosis. Treatment with hydroxychloroquine results in a regrowth of the eyelashes.[61]
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